Newswire (Published: Monday, November 16, 2020, Received: Monday, November 16, 2020, 7:03:52 PM CST)

Word Count: 512

2020 NOV 16 (NewsRx) -- By a News Reporter-Staff News Editor at Stem Cell Daily -- Investigators publish new report on Oncology - Prostate Cancer. According to news originating from Rockford, Illinois, by NewsRx correspondents, research stated, “Cancer stem cells play a major role in tumor initiation, progression, and tumor relapse of prostate cancer (PCa). Recent studies suggest that Translationally Controlled Tumor Protein (TCTP) is a critical survival factor of stem cells including cancer stem cells.”

Financial supporters for this research include NIH, United States, William E. McElroy Foundation, Brovember Inc.

Our news journalists obtained a quote from the research from the University of Illinois, “Here, we aimed to determine whether the TCTP inhibitor sertraline (STL) could target prostate cancer stem cells (PCSC). In colony formation, spheroidogenesis, angiogenesis, and wound healing assays STL showed a robust inhibition of tumorigenic (colony growth), angiogenic (endothelial tube formation) and metastatic (wound healing and migration) potential of PCSC. Interestingly, antioxidants such as N-acetyl cysteine (NAC), Glutathione (GSH) and catalase effectively blocked the cytotoxicity effect of STL on PCSC implicating oxidative stress as the underlying anti-PCSC targeting mechanism. Cell cycle analysis showed a robust G(0) arrest in PCSC exposed to STL. Notably, STL induced both apoptosis and autophagy by activating free radical generation, hydrogen peroxide formation (H2O2), lipid peroxidation (LPO) and depleted the levels of glutathione (GSH). Moreover, surface marker expression analysis using confocal revealed that STL significantly down regulates the expression levels of aldehyde dehydrogenase 1 (ALDH1) and cluster of differentiation 44 (CD44) stem cell markers. Furthermore, in western blot analysis, STL treatment applied in a dose-dependent manner, caused a marked decrease in TCTP, phospho TCTP, anti-apoptotic markers survivin and cellular inhibitor of apoptosis protein 1 (clAP1) expression as well as a significant increase in cleaved caspase3 and cleaved Poly [ADP-ribose] polymerase 1 (PARP-1) expression. Of note, STL also significantly down regulated the stem cell markers (ALDH1 and CD44) and epithelial to mesenchymal transition (EMT) markers such as transcription factor 8 (TCF8) and lymphoid enhancer-binding factor-1 (LEF1) expression levels. Concurrently, STL increased the levels of autophagy markers such as light chain (LC3), Beclin1 and autophagy-related gene (ATG5).”

According to the news editors, the research concluded: “Taken together, our study suggests that STL could be an effective therapeutic agent in eliminating prostate cancer stem cells.”

This research has been peer-reviewed.

For more information on this research see: Repurposing Antidepressant Sertraline As a Pharmacological Drug To Target Prostate Cancer Stem Cells: Dual Activation of Apoptosis and Autophagy Signaling By Deregulating Redox Balance. AMERICAN JOURNAL OF CANCER RESEARCH, 2020;10(7):2043-2065. AMERICAN JOURNAL OF CANCER RESEARCH can be contacted at: E-Century Publishing Corp, 40 White Oaks Ln, Madison, WI 53711, USA.

The news correspondents report that additional information may be obtained from Gnanasekar Munirathinam, University of Illinois, College of Medicine, Dept. of Biomedical Sciences, 1601 Parkview Ave, Rockford, IL 61107, United States. Additional authors for this research include Somaiah Chinnapaka and Velavan Bakthavachalam.

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